FLOW CYTOMETRY TO ASSESS SPERM DFI AND HDS QUALITY IN ORDER TO ASSESSTHE BEST WASHING METHOD IN OLIGOASTHENOSPERMIC PATIENTS.

Abstract

Sperm is more than just a bearer of paternal genetic information; it has a purpose much beyond fertilization. The integrity of the sperm genome is a must for the birth of healthy kids, and sperm screening should include DNA integrity testing. DNA integrity analysis is a more accurate diagnostic and predictive indicator of sperm fertility. The DNA fragmentation rate is now one of the most widely used indicators for assessing sperm fertility and predicting pregnancy outcomes. Oligospermia is a condition in which a man's sperm count is abnormally low. The condition is known as Oligozoospermia when the sperm concentration is fewer than 15 million per milliliter. This research employs Flow Cytometry (FC) to assess sperm DFI (DNA fragmentation index) quality in order to assess the best washing method in Oligoasthenospermic patients using FC Sperm Chromatin Structure Analysis (SCSA). The SCSA test is most commonly used to evaluate the percentage of sperm with fragmented DNA. Due to a lack of full protamination and consequently an elevated quantity of retained histones, the High DNA Stainable (HDS) sperm population in a semen sample has an abnormally high level of DNA staining. The different washing procedures of Swim up (SU), Density Gradient (DG), and Magnetic activated cell sorting (M) method were used and it was found that the Density Gradient Method showed low DNA fragmentation index and is suitable for Oligoasthenospermic patients for Assisted Reproductive Technology (ART) procedures.